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Technology platform

Rules of access to Technological platforms have been established and three Schemes are available for Access to Technology Platforms


 - Bursary scheme for short visits to TPs. Access scheme can be downloaded here
 Up to 200 € per visit with expectation of own contribution.

 - Technology platform visiting fellowships. Access scheme can be downloaded here
 Longer visits for up to 1 month – aimed at specific work or training. 1.000 € max. but should
 contribute approximately to one third of own costs.

 - Technology platform consumables grants. Access scheme can be downloaded here
 For short term visits with up to 3.000 € - 5.000 € maximum for consumables to be spent at TP-site.

 - Short Bio-informatics’ Training, (400.- € each)  A new Access scheme can be downloaded here. 
 (total 4800.- €) Selection by node co-ordinator.
 Bursary scheme for short visits to bio-informatic groups within MGE.

 Please follow the instructions on this form carefully. Failure to do so may disqualify your bid. One signed copy of the completed form should be sent to the Technology Platforms Committee (address below), and an electronic version e-mailed to safari@molgen.mpg.de.

Marine Genomics Network of Excellence Europe,
Technology Platforms Committee,
c/o Tamara Safari,
MPI für Molekulare Genetik,
Ihnestrasse 63,
D-14195 Berlin-Dahlem,
Germany

Platform partners


The platform partners are mentioned in the table below. For information regarding a contact name, please e-mail the web master.
The Institutions running these platforms have all agreed, either formally or in principle, that they will be made available to the NoE participants.

PlatformCapabilityCapacity

# 4 Max Planck Institute of Molecular Genetics,
Berlin
Germany

Richard Reinhardt



Highly automated genome sequencing, re-sequencing e.g. for identification of unknown SNPs and comparative sequencing, ESTs, library construction, archiving, arraying, microarrays, macroarrays, scanning of arrays, data analysis, SNP genotyping by MALDI-MS, TaqMan and SNPlex, Edman protein sequencing and MS analysis, mutation detection using WAVE technology.Up to 25,000 sequence reads per week. All other operations are on a high throughput basis and MPI should be contacted for specific details of other techniques available.

Possibility for Roche/454 capacity (TP access scheme)

One to two personnel can be trained and MPI Berlin can host visiting scientists if they are well trained.

#2 - CNRS - Roscoff Marine Station , Fr

Contact:
Erwan Corre
Sequencing facility
Genotyping facility

Platform presentation

http://www.sb-roscoff.fr/SIG/

# 22 - SZN-Stazione Zoologica Naples, It


Contact: Ina Arnone


Imaging, neuroelectrophysiology

Identification of differentially expressed genes by macroarray screening.

High throughput sequencing for screenings.
2 screenings per year if no support.
6 screenings per year if PDRAs or researchers visit and support work.


5 persons can be taken on one training course per year.
Platform presentation
# 13 University of Birmingham, UK

High throughput Sequencing Facility
microarrays, proteomics


Contact: Jim Callow




Sequencing and Genotyping
1. Daily service – users carry out their own sequencing reactions using ABI Big Dye Vers. 3 chemistry
2. Plasmid to profile – users provide DNA and the appropriate primer and the robots set up the sequencing reaction, carry out the clean up and sequence the DNA
3. Full service – users provide cultures and the lab performs the DNA extraction, the cycle sequencing reaction, clean up and sequences the samples4. Fragment analysis – AFLPs and SNPs
Microarraying
The genomics laboratory has a Biorobotics Microgrid TAS microarraying robot, capable of printing over 100 slides per run using samples stored in a temperature-controlled biobank
Samples are usually received as cDNA or Oligos.
The lab has Axon 4000A and 4000B slide scanners, which are used with Genepix 3.0 analysis software.
The genomics lab can provide facilities for spotting and scanning and advice on array design and labelling.
Proteomics
2D PAGE, Q-Tof mass spectrometry.
DNA sequencing

2-3 individuals can be trained in specific tasks.
UoB already does courses on microarraying and the use of Genespring.

Web link

#4 Max Plank Institute - Bremen, De

Contact:
Rudy Amann

Specialist laboratory for microbial molecular ecology. Specialist in environmental large fragment libraries for microorganisms. Also FISH analysis of microbial communities
Platform presentation


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